chemerin neutralizing antibody  (Bio-Techne corporation)

Journal: Cell Death & Disease

Article Title: Chemerin attracts neutrophil reverse migration by interacting with C–C motif chemokine receptor-like 2

doi: 10.1038/s41419-024-06820-5

Figure Lengend Snippet: A The concentrations of chemerin and the classic chemokines CXCL1 and CXCL2 in air pouch lavage (2 ml PBS for lavage) and plasma at 12 h, 24 h, and 48 h after LPS (2 mg/kg) stimulation. One-way ANOVA was taken out between the four groups ( n = 6). * p < 0.05. B Systemic neutralization of chemerin decreased the rM-ed neutrophil ratio in the air pouch model. Mice with air pouches received chemerin neutralization antibody by tail vein injection 24 h after LPS stimulation. After 6 h, blood cells were collected, and the rM-ed neutrophil ratio was determined. MFI Mean fluorescence intensity. * t -test was taken out between the two groups ( n = 3). * p < 0.05.

Article Snippet: In the air pouch model, at 24 h after LPS injection into the air pouch, the mice were administered an anti-chemerin antibody (10 μg, MAB2325, R&D Systems) by tail vein injection to neutralize chemerin in the circulation.

Techniques: Clinical Proteomics, Neutralization, Injection, Fluorescence